Accelerated Titering of Adenoviruses

Production and Titering of Recombinant Adeno-associated Viral Vectors

In recent years recombinant adeno-associated viral vectors (AAV) have become increasingly valuable for in vivo studies in animals, and are also currently being tested in human clinical trials. Wild-type AAV is a non-pathogenic member of the parvoviridae family and inherently replication-deficient. The broad transduction profile, low immune response as well as the strong and persistent transgene...

Titering replication-defective virus for use in gene transfer.

Characteristics of the Adenoviruses

The strains of types 1 to 4 adenoviruses studied attained maximum combination with host cells, strain HeLa, in 5 to 6 hours. During this adsorption period approximately 75 per cent of the total virus became associated with the host cells. Following adsorption of virus to HeLa cells, these agents underwent cyclic multiplication periods similar to bacterial and other animal viruses. The latent or...

Avian adenoviruses.

Adenovirus infections are ubiquitous in commercially farmed birds, and probably in all avian species. There is a wide range of virulence, in some cases even within the same serotype. While many infections are subclinical and appear to be of little economic or welfare importance, significant outbreaks of disease associated with adenovirus do occur. These diseases are not of public health signifi...

Ophthalmologists beware of adenoviruses

Epidemic keratoconjunctivitis caused by different serotypes of human adenoviruses is an explosive and highly contagious ocular surface infection. This is the case of a young ophthalmologist who presented a filamentary keratitis complicating an adenoviral keratoconjunctivitis. Filamentary keratitis is a chronic and debilitating disorder related to dry eye syndrome. Patients with filamentary kera...